Journal: Discover. Oncology

Article Title: CC chemokine receptor 7 promotes macrophage recruitment and induces M2-polarization through CC chemokine ligand 19&21 in oral squamous cell carcinoma

doi: 10.1007/s12672-022-00533-x

Figure Lengend Snippet: Primers used for qRT-PCR

Article Snippet: A mouse monoclonal anti‑CD163 antibody (cat No: sc‑33715) from Santa Cruz (Dallas, TX, USA) was utilized for immunofluorescence staining.

Techniques:

Journal: Discover. Oncology

Article Title: CC chemokine receptor 7 promotes macrophage recruitment and induces M2-polarization through CC chemokine ligand 19&21 in oral squamous cell carcinoma

doi: 10.1007/s12672-022-00533-x

Figure Lengend Snippet: Knockdown of CCR7 in OSCC cells suppressed macrophage M2-polarization. A Representative morphological images of THP-1 derived macrophages cultivated with OSCC CM. The cytoskeleton was labelled with Actin-Tracker Red-555 Fluorescent Phalloidin (red), and nuclei were stained with DAPI (blue) (scale bar: 50 μm). B The mRNA expression level of CD163, CD206, IL-10 and TGF-β1 of macrophages co-cultured with OSCC CM, evaluated by qRT-PCR. C Representative Scatter plots showed the CD206 expression level of macrophages incubated with OSCC CM, measured by flow cytometry. The histogram displayed statistical analysis for the proportion of CD206+ macrophages. D Representative fluorescent images of macrophages treated with OSCC CM. The cytomembrane CD163 was labelled with Alexa Fluor® 488 (green), and nuclei were stained with DAPI (blue) (scale bar: 50um). The results are presented as the mean ± SD, ns: not significant, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: A mouse monoclonal anti‑CD163 antibody (cat No: sc‑33715) from Santa Cruz (Dallas, TX, USA) was utilized for immunofluorescence staining.

Techniques: Derivative Assay, Staining, Expressing, Cell Culture, Quantitative RT-PCR, Incubation, Flow Cytometry

Journal: Discover. Oncology

Article Title: CC chemokine receptor 7 promotes macrophage recruitment and induces M2-polarization through CC chemokine ligand 19&21 in oral squamous cell carcinoma

doi: 10.1007/s12672-022-00533-x

Figure Lengend Snippet: CCL19 and CCL21 promoted macrophage M2-polarization in vitro. A Spearman correlation analysis of CCR7 expression with CCL19 and CCL21, based on TIMER2.0 database. B Correlation between CCR7 expression and M2 macrophage, based on TIMER2.0 database. C The mRNA expression level of CD163, CD206, IL-10 and TGF-β1 of THP-1 derived macrophages stimulated by 400 ng/ml recombinant human CCL19 and CCL21, evaluated by qRT-PCR. D Representative Scatter plots showed the CD206 expression level of macrophages incubated with 400 ng/ml recombinant human CCL19 and CCL21, measured by flow cytometry. The histogram displayed statistical analysis for the proportion of CD206+ macrophages. The results are presented as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: A mouse monoclonal anti‑CD163 antibody (cat No: sc‑33715) from Santa Cruz (Dallas, TX, USA) was utilized for immunofluorescence staining.

Techniques: In Vitro, Expressing, Derivative Assay, Recombinant, Quantitative RT-PCR, Incubation, Flow Cytometry